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| Registros recuperados: 54 | |
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| Dzionek,Anna; Wojcieszyńska,Danuta; Guzik,Urszula. |
| Bioremediation of contaminated groundwater or soil is currently the cheapest and the least harmful method of removing xenobiotics from the environment. Immobilization of microorganisms capable of degrading specific contaminants significantly promotes bioremediation processes, reduces their costs, and also allows for the multiple use of biocatalysts. Among the developed methods of immobilization, adsorption on the surface is the most common method in bioremediation, due to the simplicity of the procedure and its non-toxicity. The choice of carrier is an essential element for successful bioremediation. It is also important to consider the type of process ( n s tu or ex s tu), type of pollution, and properties of immobilized microorganisms. For these reasons,... |
| Tipo: Journal article |
Palavras-chave: Adsorption; Bioethanol production; Contamination; Heavy metals; Immobilization; Immobilized microorganisms; Natural carriers; Wine alterations; Wood barrels; Xenobiotics. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500008 |
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| Xiao,Anfeng; Xu,Caiyun; Lin,Yan; Ni,Hui; Zhu,Yanbing; Cai,Huinong. |
| Background Carboxyl-functionalized magnetic nanoparticles were synthesized via chemical co-precipitation method and modified with oleic acid which was oxidized by potassium permanganate, and κ-carrageenase from Pseudoalteromonas sp. ASY5 was subsequently immobilized onto them. The immobilization conditions were further optimized, and the characterizations of the immobilized κ-carrageenase were investigated. Results The κ-carrageenase was immobilized onto magnetic iron oxide nanoparticles, and the bonding was verified by Fourier transform infrared spectroscopy. The optimal conditions for κ-carrageenase immobilization were 2.5% (w/v) glutaraldehyde, 13.9 U κ-carrageenase for 20 mg of magnetic nanoparticles, a 2-h... |
| Tipo: Journal article |
Palavras-chave: Carrageenase; Characterization; Immobilization; Nanoparticles. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100001 |
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| Ibrahim,Abdelnasser S.S.; Al-Salamah,Ali A; El-Toni,Ahmed Mohamed; El-Tayeb,Mohamed A; Elbadawi,Yahya B. |
| Background Cyclodextrin glucanotransferase (CGTase) from Amphibacillus sp. NPST-10 was covalently immobilized onto amino-functionalized magnetic double mesoporous core-shell silica nanospheres (mag@d-SiO2@m-SiO2-NH2), and the properties of the immobilized enzyme were investigated. The synthesis process of the nanospheres included preparing core magnetic magnetite (Fe3O4) nanoparticles, coating the Fe3O4 with a dense silica layer, followed by further coating with functionalized or non-functionalized mesoporous silica shell. The structure of the synthesized nanospheres was characterized using TEM, XRD, and FT-IR analyses. CGTase was immobilized onto the functionalized and non-functionalized nanospheres by covalent attachment and physical adsorption. Results... |
| Tipo: Journal article |
Palavras-chave: Amphibacillus sp; Cyclodextrin glucanotransferase; Cyclodextrins; Double mesoporous core-shell silica nanospheres; Immobilization. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200001 |
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| Nawani,Neerupma; Singh,Rajvinder; Kaur,Jagdeep. |
| A thermostable lipase was partially purified from the culture supernatant of a thermophilic Bacillus sp. The enzyme is optimally active at 60ºC and pH 8.0. The enzyme showed enhancement in activity in presence of benzene or hexane (30% v/v each). The activity (assayed by determining the release of pNP from pNP laurate) was stimulated up to 60% of these solvents in enzyme reaction mixture. The catalytic properties of this thermostable enzyme can be further improved via the use of different immobilization techniques and reaction conditions. Enzyme was immobilized on different solid supports and their enzyme activity and stability was compared. The enzyme was adsorbed on silica and HP-20 beads followed by cross-linking with gluteraldehyde on HP-20,... |
| Tipo: Journal article |
Palavras-chave: Esterification; Immobilization; Lipase; Thermostability. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500011 |
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| Jin,Yuan-yuan; Li,Ya-dong; Sun,Wan; Fan,Shuai; Feng,Xiao-zhou; Wang,Kang-you; He,Wei-qing; Yang,Zhao-yong. |
| Background: D-Hydroxyphenylglycine is considered to be an important chiral molecular building-block of antibiotic reagents such as pesticides, and β-lactam antibiotics. The process of its production is catalyzed by D-hydantoinase and D-carbamoylase in a two-step enzyme reaction. How to enhance the catalytic potential of the two enzymes is valuable for industrial application. In this investigation, an Escherichia coli strain genetically engineered with D-hydantoinase was immobilized by calcium alginate with certain adjuncts to evaluate the optimal condition for the biosynthesis of D-carbamoyl-p-hydroxyphenylglycine (D-CpHPG), the compound further be converted to D-hydroxyphenylglycine (D-HPG) by carbamoylase. Results: The optimal medium to produce... |
| Tipo: Journal article |
Palavras-chave: Calcium alginate; D-Carbamoyl-p-hydroxyphenylglycine; D-Hydantoinase; D-Hydroxyphenylglycine; Immobilization; Whole cell. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300004 |
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| Landreau, M.; Duthoit, Frederique; Claeys-bruno, M.; Vandenabeele-trambouze, O.; Aubry, T.; Godfroy, Anne; Le Blay, Gwenaelle. |
| Aims The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro-organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures. Methods and Results The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5-week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (<0∙5 mol l−1) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect... |
| Tipo: Text |
Palavras-chave: (hyper)thermophilic marine micro-organisms; Anaerobiosis; Entrapment; Gellan; Immobilization; Xanthan. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00332/44352/44000.pdf |
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| Landreau, M.; Duthoit, Frederique; Roussel, Erwan; Schonherr, S.; Georges, Myriam; Godfroy, Anne; Le Blay, G.. |
| Cultivation in a bioreactor of immobilized deep-sea hydrothermal microbial community was tested in order to assess the stability and reactivity of this new system. A community composed of 8 hydrothermal strains was entrapped in a polymer matrix that was used to inoculate a continuous culture in a gas-lift bioreactor. The continuous culture was performed for 41 days at successively 60°C, 55°C, 60°C, 85°C and 60°C, at pH 6.5, in anaerobic condition and constant dilution rate. Oxic stress and pH variations were tested at the beginning of the incubation. Despite these detrimental conditions, 3 strains including 2 strict anaerobes were maintained in the bioreactor. High cell concentrations (3 × 108 cells mL−1) and high ATP contents were measured in both liquid... |
| Tipo: Text |
Palavras-chave: Immobilization; Anaerobiosis; Marine (hyper)thermophiles; Continuous culture. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00347/45851/45516.pdf |
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| Fonseca, E. L.; Núñez, R. R.; Villaverde, M. J; Barbán, O. M.; Ferrer, O. B.; Ramos, I.; Valdés, O.. |
| Una marea negra provocada por el vertido deliberado o accidental de crudos de petróleo, aceite combustible, fangos o residuos petrolíferos y productos refinados, provoca un gran impacto sobre los ecosistemas marinos y costeros. En este trabajo, se desarrolla un producto compuesto por bacterias marinas inmovilizadas por atrapamiento en k- carragenina semirrefinada y en cuya formulación no se incluyen nutrientes. Los resultados muestran que la variante de inmovilización más ventajosa se obtuvo cuando fue empleada la κ-carragenina al 1% gelificada con una solución de KCL 1.5 M. Mediante análisis de Cromatografía Gaseosa de Alta Resolución y de espectroscopia (FTIR), se demostró la alta capacidad de degradación del bioproducto frente a petróleo del Yacimiento... |
| Tipo: Preprint |
Palavras-chave: Immobilization; Oil; Immobilization; Http://aims.fao.org/aos/agrovoc/c_27550. |
| Ano: 2006 |
URL: http://hdl.handle.net/1834/3662 |
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| Santos, Danilo Rheinheimer; UFSM; Martinazzo, Rosane; UFRGS; Gatiboni, Luciano Colpo; UDESC; Kaminski, João; UFSM; Silva, Leandro Souza da; UFSM. |
| Em solos com elevado grau de intemperismo, a imobilização temporária de fósforo pela biomassa microbiana do solo (BMS) pode ser um mecanismo de retardamento das reações de adsorção. O objetivo do presente trabalho foi avaliar a amplitude no fósforo microbiano (Pm) em um Argissolo em pastagem nativa submetida à roçada, introdução de forrageiras exóticas e adubadas com fertilizantes fosfatados. O estudo foi desenvolvido a partir de um experimento instalado em 1997 que constava de cinco tratamentos de fosfato e calagem (testemunha sem fósforo; hiperfosfato de gafsa; superfosfato triplo; superfosfato triplo + calcário e superfosfato simples + calcário), distribuídos em quatro blocos ao acaso. Em junho de 2002, a pastagem nativa remanescente foi roçada, semeada... |
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Palavras-chave: 5.01.00.00-9 Agronomia biomassa microbiana; Imobilização; Adubos fosfatados; Pastagens nativas; Disponibilidade de fósforo. microbial biomass; Immobilization; Phosphate fertilizers; Natural pasture; Phosphorus availability. |
| Ano: 2008 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5319 |
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| PEREIRA,ANDRESSA R.; SEDENHO,GRAZIELA C.; SOUZA,JOÃO C. P. DE; CRESPILHO,FRANK N.. |
| ABSTRACT Bioelectrochemistry can be defined as a branch of Chemical Science concerned with electron-proton transfer and transport involving biomolecules, as well as electrode reactions of redox enzymes. The bioelectrochemical reactions and system have direct impact in biotechnological development, in medical devices designing, in the behavior of DNA-protein complexes, in green-energy and bioenergy concepts, and make it possible an understanding of metabolism of all living organisms (e.g. humans) where biomolecules are integral to health and proper functioning. In the last years, many researchers have dedicated itself to study different redox enzymes by using electrochemistry, aiming to understand their mechanisms and to develop promising bioanodes and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioelectrocatalysis; Biofuel cells; Bioelectrochemistry; Immobilization; Protein-electrode interactions; Redox enzymes. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000200825 |
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| Durigan,João Luiz Quagliotti; Cancelliero,Karina Maria; Bosi,Paula Lima; Delfino,Gabriel Borges; Montebelo,Maria Imaculada de Lima; Guirro,Rinaldo Roberto de Jesus; Silva,Carlos Alberto da; Polacow,Maria Luiza Ozores. |
| The aim of this work was to investigate the effect of the neuromuscular electrical stimulation (ES) on the metabolic and morfometric profile of the tibialis anterior muscle, antagonist to the soleus muscle which was stimulate, under the joint immobilization condition of the ankle for 7 and 15 days. The immobilization promoted the reduction in the muscle mass (I7: 17.36%, I15: 20.83%), in the glycogen content (I7: 48%, I15: 48%), in the muscle fibers areas (I7: 27%, I15: 40%) and increase in intramuscular connective tissue density (I7: 122%, I15: 206%). The EE didn't promote significant alterations in the mass of the immobilized groups, however, promoted increase in the glycogen (IEE7: 31.25%; IEE15: 56.25%), reduction in the muscle fibers areas (IEE7: 14%,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Immobilization; Muscle disuse; Metabolic; Morphometric; Neuromuscular electrical stimulation; Rehabilitation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100011 |
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| Registros recuperados: 54 | |
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